HPTLC and HPLC Analysis of Bioactive Phyllanthin from Different Organs of Phyllanthus amarus

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Physicochemical characterization of phyllanthin from Phyllanthus amarus Schum. et Thonn.

Phyllanthin is a major bioactive lignan component of Phyllanthus amarus, with several known biological activities. This study dealt with the isolation and physicochemical characterization of phyllanthin. Phyllanthin was isolated from P. amarus leaves by column chromatography and purified by recrystallization to obtain phyllanthin crystals with a purity of more than 98%. UV, IR, MS, (1)H NMR and...

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Isolation of Rutin from Phyllanthus Amarus

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Optimization of process variables for phyllanthin extraction from Phyllanthus amarus leaves by supercritical fluid using a Box-Behnken experimental design followed by HPLC identification.

The response surface methodology using the Box-Behnken design was established to describe supercritical carbon dioxide assisted extraction of phyllanthin from Phyllanthus amarus Schum and Thonn leaves prior to HPLC analysis. The effects of extraction pressure, temperature, modifier concentration and extraction time on the yield of phyllanthin were investigated. By solving the regression equatio...

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Phyllanthus Amarus: A Review

Herbs have always formed an integral part of human health and are used in the treatment of several human diseases. One of such species which have wide patronage of use is Phyllanthus amarus. This herb is in traditional medicine for more than 3,000 years. The plant has also served as lead for several experimental investigations that explored its phytochemical constituents and pharmacological use...

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Preparation and Characterization of Phytosomal-Phospholipid Complex of P. Amarus and its Tablet Formulation

Present investigation was aimed at formulation, characterization and evaluation of phytosomal complex tablets for sustained delivery of Phyllanthus amarus complex. Phyllanthin, one of the active lignin present in this plant species was isolated from the aerial parts, by silica gel column chromatography employing gradient elution with hexane–ethyl acetate solvent mixture. It was obtained in high...

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ژورنال

عنوان ژورنال: Asian Journal of Biotechnology

سال: 2009

ISSN: 1996-0700

DOI: 10.3923/ajbkr.2009.154.162